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However, it is difficult to delete genes in Clostridium as the genomic integration remains a challenge [24], [36], [46].
However, due to the finite number of resistance genes available in Geobacter, it is difficult to delete additional loci or complement such complex constructs (10).
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Where once a spurned lover could use scissors (literally) to cut an ex out of the picture, digital images of the smiling couple in happier days abound on the Web and are difficult to delete.
Although eqxC has been previously deleted, eqxS was difficult to delete.
Because the endogenous IgH locus is beset with redundant regulatory elements, it is difficult to identify the direct effects of deleting the enhancer.
It is difficult to detect a small fraction of deleted mutant genomic DNA in the presence of a vast excess of wild type DNA with array CGH or other popular molecular biology tools [26], [27], [35].
It is difficult to quantify how many Facebookers are frustrated enough to hit the delete button and go searching for greener pastures.
It is difficult to watch.
"It is difficult to get her appointment.
"It is difficult to say," he said.
It is difficult to have hope.
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