We found that 10 mM EGTA was indeed very effective at increasing the duration of our experiments, and it is critical to observing micro-domains of Ca2+ sparklets since the on rate of Fluo 4 is higher than the on rate of EGTA, resulting in a localization and improved kinetic resolution of Ca2+ sparklet signal (Shuai and Parker, 2005).
Such a micro-temporal level approach is critical to observing the variation of weather over the course of the day, and over other temporal scales.
In order to derive a mechanistic understanding of how gene transcription is regulated over the course of RA - for example, in response to therapeutic agents - it will be critical to observe these changes over time in specific cell types, preferably in conjunction with a simultaneously obtained gene expression profile.
It was determined that bulky substituents α to the original position of the tosylate were critical to observe high es in this substitution reaction.
Much of this paper will describe the precise rules for UKA positioning, which are critical to observe to warrant these implants outcome and longevity.
We would like to emphasize that the size and shape of the courtship chamber are critical to observe the decamping of the female and subsequent searching behavior of the male (i.e., visual tracking versus scanning).
I realized from observing others that it was critical to find one's focus and voice and have it heard quickly.
It is critical to evaluate the reliability of observing a given connection across multiple injections.
Developmental timing of hormone exposure is critical to the type of response observed in the seedlings.
Indeed, when comparing the present results to other experimental designs, it will be critical to note that these findings were observed in male subjects while viewing female faces.
