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The GS-FLX Titanium system was chosen because it generates longer sequence length (~ 400 bp) per read than most other next generation sequencing systems, which is important for the subsequent design of SSR primers flanking the microsatellite motifs.
For the de novo transcriptome sequencing we chose Roche 454 pyrosequencing because it generates longer reads as compared to the Illumina technology [ 16].
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Morning fasting generates longer and more intense evening grazing bouts, increasing the DMI of higher nutritive herbage, making cattle perform equally in shorter grazing sessions.
We then present our modified SAGE procedure that generates longer sequence tags (14 bp) rather in detail, and the profile (80K profile) derived from HeLa cells that is composed of 80 000 tags obtained from a single library.
On such libraries exSPA nder and ALLPATHS-LG generate comparable results (exSPA nder generates longer contigs but ALLPATHS-LG generates longer scaffolds).
This technology generates longer transcripts than Illumina, facilitating de novo assembly [ 31].
The assembly generates longer sequences, which gives a better chance of association of sequences with the proteins.
SSS mutations cluster around the sole integrin (RGD -binding site of fibRGD -bindingereasitee Tsk mutatiof generates longer fibrillin-1 molecules whereasplicathe RGD sequences [ 37, 43].
High throughput 454 sequencing which generates longer reads has been widely used in many transcriptome sequencing studies in non-model species previously [ 48, 49].
A one-time price increase can create a substantial short-term lift in revenue, but is it worth it if it generates a longer term fear in users?
GapFiller is a seed-and-extend method able to correctly fill the gap between paired reads, thus it generates accurate longer sequences with respect to input reads.
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