To make it Gateway-compatible, a Gateway cassette was introduced into the multiple cloning sites located between the Ubi promoter and the Nos 3' terminator.
The not-so-happy optimistic view is that without it, Gateway's net loss would have been 50% wider.
To facilitate this analysis, we modified the p2T7-177 inducible RNAi vector [29] to make it Gateway® compatible.
This system was further refined by making it Gateway (Invitrogen) cloning compatible, allowing researchers to combine any promoter, coding sequence, and 3′-UTR to create expression vectors (Stoltzfus et al. 2014).
Barry Sheene used to call it "the gateway to hypothermia" and it can be very cold when the wind whips in.
But don't think beer geeks won't enjoy it, and don't call it a "gateway beer".
Its mainstream-friendly price point and accessibility also make it the gateway drug that VR needs.
But I consider it a gateway to bigger crimes, the way they say marijuana leads to heavier drugs.
Gateway Acquisition Helps Acer; But Will it Help Gateway? [Wired Blogs] .
