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For years, the IT epicentre has been South India's unofficial bar capital, a bustling city of chic rooftops, hole-in-the-wall bars, and dozens of venues in between.
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Approximately 250 ng of mononucleosomal DNA was end-cleaned with END-it (Epicentre) in a 40 μl reaction.
Geoscience Australia said it could have been felt as far as 500km from its epicentre, although any damage would be limited to a 40km radius.
Its epicentre was just 15 miles south-west of Port-au-Prince and it began only eight miles below ground.
With a magnitude of 7.8, it was the worst to hit the country for more than 80 years, its epicentre 50 miles north-west of the capital, Kathmandu.
It is a mile from the centre of the town, however, where the Che legend has its epicentre.
It was one of many aftershocks to follow the 11 March Tōhoku earthquake, and the strongest to have its epicentre located inland.
Briefly, DNA ends were polished using the End-It kit (Epicentre) and then purified using MinElute reaction cleanup column (Qiagen).
After fragmentation, the fragments were blunt-ended and phosophorylated at the 5' end using the End-it Kit (Epicentre) according to the manufacturer's instructions.
DNA fragments isolated from ChIP were repaired to blunt ends by T4 DNA polymerase and phosphorylated with T4 polynucleotide kinase using the END-IT kit (Epicentre, Madison, WI).
After fragmentation, fragments were blunt-ended and phosophorylated at 5'-prime end using End-it Kit (Epicentre) according to the manufacturer's instructions.
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CEO of Professional Science Editing for Scientists @ prosciediting.com