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His method is avant-garde because it eliminates the sequencing of DNA to keep the analysis cheap and quick.Before a scrap of DNA can be sequenced, it needs to be extracted from a cell and then copied many times to create enough for experiments.
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We searched the IMGT database (http://imgt.cines.fr/) to identify the human VH and VL sequence pairs, which are most homologous to the corresponding variable regions of m357, while eliminating the sequences of phage-display or humanized antibodies from our search results.
Simrank eliminates the effect of sequence discrepancies arising solely from homopolymer exaggeration.
Assuming that the reddening associated with the Nellore allele was recessive, the interval could be refined to a region between 71708424 and 72478577 bp on BTA6, thereby eliminating the coding sequences of KIT, KDR and CORIN as candidates.
Constraining the sets to these sequence motifs eliminates the possibility of selecting palindromic sequences that could form a stable homoduplex and ligate, which terminates the encoding DNA by virtue of displaying the incorrect overhang for subsequent ligation.
SeqZip has no RT step, it eliminates long intervening regions of common sequence, and the unique exon-specific barcodes introduced during the ligation step further discourage template switching during subsequent amplification.
The use of consensus sequences eliminates the possibility of assessing potential distance thresholds for identification and allows only the use of characters that are shared by all members of a species for identification.
Deletion of the consensus R164 K165 K166 R167 sequence eliminates the cleavage of PA by furin-like proteases and by trypsin [ 22].
This design eliminates the presence of repeat sequences derived from the target gene.
Alternative design of oligonucleotides that removes sequence redundancy eliminates the formation of both antisense and double-stranded RNA species.
This not only reduces the cost associated with genome sequencing but also eliminates the need of generating NT lines for each chromosome of the polyploid genome.
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