Additionally, the WRJ and WRK primer pair was chosen because it does not amplify the P1-vv and P1-rr-4B2 alleles.
This novel isoform was detected in 1/62 subclones from brain, and amplified at low levels throughout the nervous system and embryonic tissues, with primer pairs DIC1_Ex 1 for and DIC1_R rev, and DIC1_Ex 1 for and DIC1_iso14 rev, but it does not amplify in other tissues.
Separate PCRs should use (1) unlabeled forward and reverse primers to make sure they are designed well and amplify fragments; (2) the forward tailed primer and the reverse primer only to make sure they also amplify fragments; (3) the labeled tail alone to verify that it does not amplify any product.
It also brings to attention the fact that a small fraction of confounding would be expected to exist, attributable to the residual imbalance in the included covariates (and the correlated fraction of their nonincluded covariates), that is not detected because it does not amplify.
An ISBP primer pair was considered 3AS specific if it did not amplify a product from N3AT3B or Dt3AL but amplified products from all other stocks.
Furthermore the newly designed duplex real-time RT-PCR proved to be strictly AHSV-specific as it did not amplify close related viruses.
The assay was specific in that it did not amplify other porcine viruses including porcine parvovirus, porcine circovirus type 1, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus, classical swine fever virus, swine transmissible gastroenteritis coronavirus, and porcine epidemic diarrhea virus.
It is likely that this sample carries very low AdV particle number, as it did not amplify by RQ PCR.
The method was highly specific since it did not amplify DNA from other bacterial species that can potentially cause epididymitis in rams as well as species phylogenetically related to B. ovis.
If that doesn't amplify it enough to your liking, run a "compressor" or "hard limiting" on it, which can make soft portions loud while keeping loud portions from clipping any further.
"He doesn't amplify his violin, he plays the notes as written.
