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The protein coded by Med14Y would be highly homologous to the X-chromosome Med14 (Additional file 1: Figure S9) if it could be transcribed and translated.
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The human genome is constituted by protein-coding sequences for only two percent while the remaining vast majority of it, previously considered as "junk DNA," could be transcribed and it is now named as non-coding RNA (ncRNA).
Therefore, when larger DNA fragments are excised containing entire genes that potentially could be transcribed, it may be desirable to follow the fate of the excised fragment.
One explanation is that an IS element transposed into an operon and separated it into two parts that could be transcribed individually.
It is possible that these domains could be transcribed from a distinct DNA locus and the rRNA reassembled post-transcriptionally.
Finally, if the candidate sequence could be transcribed and if it was not the exon of a protein-coding gene, then it was assumed to be a miRNA precursor.
It was a stretch of DNA that could be transcribed by an enzyme called polymerase into a chemically similar molecule known as RNA.
It is possible that a small part, or even a particular SINE copy (master or source gene), could be transcribed and its RNA potentially used for retropositioning [6], [10].
In aggregate, these data demonstrated that the DUX4c gene could be transcribed from its natural promoter into RNAs covering its entire ORF, and that such mRNAs were expressed in FSHD and control myoblasts.
It was found that most of the human genome could be transcribed into RNAs but only a small fraction of these RNAs was translated into proteins (Cheng et al. 2005), i.e., most RNAs did not undergo translation.
It is proposed that some genes that belong to multigene families could be transcribed and function as siRNAs.
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