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When 20E peaks, it binds with its heterodimeric receptors EcR and USP to initiate the early genes, such as the set of transcription factors EcR, USP, E74, E75, BR-C, HR3, and HR4 [15], and is followed by several relative late genes, including proteinases [16], [17] and other late genes [18], [19].
When sRNA is expressed it binds with its mRNA target at a rate k R and quickly degrades thereafter.
This probe displays a 25-fold increase in fluorescence upon binding Ni2+, although it binds with relatively low affinity (Kd = 193 μM).
These stimuli can prompt the active NF- κB (p65 p50 subunits) translocate into the nucleus where it binds with NF- κB-specific DNA-binding sites to activate transcriptionally the expression of several survival genes.
We have used stopped-flow analysis to assess the rate of binding of phospho Ets to ERK2 and found that it binds with a rate that exceeds the rate of diffusion for the association of two proteins, with a rate constant for association of kon = 5×106 M-1s-1 M-1s-1 M-1s-1 constand for dissociation of k >100 s-1 [13].
Our data suggests that NtcA could act similarly in those sites where it binds with a low affinity and for which no consensus-binding site can be found.
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In plasma, it bound with lipoproteins and albumin which facilitated its carrier-mediated cellular uptake [ 41].
Biochemical analysis of γ-TuSCSpc98Δ1 156 showed that its binding to Spc1101–220-5D and oligomerization capability by Spc1101–220-5D were reduced, although in M phase it bound with the same affinity for SPBs as WT γ-TuSCSpc98Δ1 156
To soften the impact, Aykroyd made a joke of the thick script and had it bound with the cover of the Los Angeles Yellow Pages directory for when he turned it in to producer Robert K. Weiss.
Heteromerization played a key role in the presynaptic profile of SCH-442416, since it bound with much less affinity to A2AR when co-expressed with D2R than with A1R.
It bound with high affinity to the human adenosine A1 receptor in this transfected cell system (log KD−7.38).
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