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Furthermore, when PCRs are multiplexed, it becomes difficult to check successful amplification per fragment, the chance of obtaining artifacts increases and equimolar loading before sequencing becomes very difficult.
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However, it becomes difficult to see the patterns described above.
At this point, it becomes difficult to hear, because Def Leppard is giving a sound check that literally shakes the floors.
It becomes difficult to maintain faith.
It becomes difficult to decipher what style and dressy is".
When that is compromised, it becomes difficult to win".
It becomes difficult to study old geomagnetic events.
Energy consumption becomes very crucial when it comes to underwater, as it becomes difficult to replace/charge the sensor nodes.
It becomes difficult to ignore such kind of special offers.
1) It becomes difficult to experience dependency without shame.
It becomes difficult to move.
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