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C-terminal tagged DAYSLEEPER appeared non-functional, since it did not complement the daysleeper phenotype.
The secondary structure and folding analysis of the remaining seven sequence types identified a potential pseudogene that appeared non-functional (data not shown) and this was also dropped from the analysis.
The presence of flagellar biosynthesis genes that appear non-functional within bacterial genomes has been observed previously [ 61] and is suggestive of evolved mechanisms for evasion of host detection as flagellar filament proteins serve as antigenic triggers of host immune responses [ 62, 63].
Thus, by sequence analysis, it appears that non-mutated NUMTs are functional to promote replication while it is not clear, at least with the present set of data, whether point mutations in the ACS motifs or in the entire NUMTs affect the replication properties of these sequences.
Nineteen other mutant proteins (30%) appeared totally non-functional.
We next interrogated why caspase-10B and -10G appeared either non-functional or having an opposite role compared with isoforms A and D. The absence of the proteolytic domain in the caspase-10G isoform is sufficient to explain its inability to stimulate DR-induced apoptosis.
You can't make it smaller without making it non-functional — so make it bigger.
Firstly, it also appears to be non-functional; about 780 bp upstream of the intein insertion site, the RNA polymerase coding sequence contains a frameshift mutation and there is a nonsense mutation six codons upstream of the putative intein.
This is a paradox because most of the DNA appears to be non-functional while most of the externally observable phenotype appears to have adaptive function.
As she says of the Swimming Reindeer: "The crucial point of this piece is that it is non-functional.
TRMV-OR2 appears to be non-functional and has no leader peptide, but it does contain a complete V gene segment and the recombination signal sequence (RSS).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com