Sentence examples for it 1st from inspiring English sources
There are infinite levels to play it: 1st level will be 5 punches with palm, 2nd level will be 10, 3rd will be 15 and so on, adding 5 each level.
https://t.co/lByfHdE32p @EnglandRugby is it 1st of April already?
The activation became maximal at a site where it 1st appeared, at the location of the border between areas 17 and 18.
As shown in Figures 3(a) and 3(b), Tamoxifen or Gefitinib alone significantly suppressed the colony forming capacity of PC9 cells (1st versus 2nd or 3rd well/bar), and Gef plus TAM further suppressed it (1st versus 4th well/bar).
I never said @amyschumer stole my joke.I just said it was the same.It's possible we both wrote it.I just wanted you to know I wrote it 1st.
Said he No cake shall go to the prisoner without me inspecting it 1st and he waved his big soft white hand thus indicating my mother should place her basket on his desk.
Total RNA (1 μg) was reverse transcribed (Reverse-iT 1st strand synthesis kit, ABgene, Epsom, UK) and amplified for XBP1/XBP1s and housekeeping β-actin (3 and 1.5 μl cDNA, respectively).
Total RNA was extracted from cells (EZ RNA kit, Biological Industries, Beit Haemek, Israel) and reverse transcribed to cDNA using Reverse-iT 1st Strand Synthesis Kit (ABgene House, AB-0789) and oligo-dT primer (Takara Shuzo Co. Ltd., Seta, Japan).
cDNA was synthesized using a Reverse-iT 1st Strand Synthesis Kit (ABgene, Epsom, UK).
