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Regardless of the potential action of miR3522 on this family of retroelements, the high abundance of miR3522 is not tied to the repeated nature of the retroelement's LTR ends and it's sequence is even more degenerate in other locations and therefore, this extremely abundant miR3522 sequence was removed from the size distribution analysis of small RNAs matching to transposons shown in Figure 7.
To remedy this, we removed any EST that had less than 90% of it's sequence included in the BLAST alignment.
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I had a real moment playing it out last year Panorama Bar's NYE celebration just before Virginia, and the way it's sequenced gives you that euphoric reaction at least three times.
Like the Harry Potter books it is sequence of seven novels with a boy magician at its heart.
Multilocus sequence typing of K. pneumoniae N10 0469 showed that it was sequence type (ST) 16 (16 ).
"There is only one human genome," says Tachi Yamada, the new group's head of R&D, "once it is sequenced, that is it.
When the DGE-tag library has been constructed, it was sequenced by Illumina Cluster Station and Genome Analyzer.
The first 5 bases of each read will identify the clone within the batch from which it was sequenced.
Once a positive individual is found it is sequenced to determine the exact mutation it carries.
aFrequency could be underestimated because it was sequenced only in patients with obvious symptoms.
Once the plasmid was isolated, it was sequenced by Eurofins Genomics (UK).
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