It was probed with 5′ end P-labeled oligonucleotide probes against hsa-miR-122 at 37°C for 16 hr in a hybridization oven.
This topic came up spontaneously in the first focus groups and it was probed in the following group interviews if it did not arise spontaneously again.
Further, it was probed whether the lack of availability of HIT applications was indicated by the professionals to be one of the reasons for shifting jobs.
Once an issue emerged as being important in an interview (such as feeling frustrated with the healthcare system), it was probed for in all subsequent interviews.
This has opened the way for the CMAP to be used as a drug discovery tool where it is probed with signatures encoding disease states.
After transfer to nitrocellulose membranes, it was probed with anti-IGFBP5 antibody (diluted 1 1000 in TBST, 5% non-fat dry milk; Santa Cruz Biotechnology, Santa Cruz, CA).
After treating the membrane with stripping buffer (0.1 m glycine, pH 2.3), it was probed using the antibodies that recognize the total amount of the specific protein of interest.
Shares in the brokerage were down 84 cents, or 0.5%, to $155.07, after the New York attorney general's office said it is probing whether the firm gave Fidelity Investments incentives to sell auction-rate securities.
Their long curved bills fit the tube-like flowers exactly, so that the pollen-presenter brushes against the spinebill's head while it is probing for nectar.
