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If it's homogenized, you're out of luck.
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It was homogenized in 10 volumes of homogenization buffer consisting of 0.1M, pH 7.4 Tris-HCl (0.1M, pH 7.4) containing DTT (1 mM), KCl (1.15% v/v), PMSF (0.1 mM), BHT (22 mM), aprotinin (0.001% w/v), leupeptin (0.001% w/v) and glycerol (20% v/v).
Further, it was homogenized using mortar pestle.
It was homogenized again when the solution was poured and the film was cast in Pyrex dish.
In the prone group (B), ventilation distribution is significantly different within the group at ALI_SP/PP and PEEP 10 and 10d, whereas it is homogenized at PEEP 20, 30 and 20d.
How is it being homogenized?
For tissues and droppings, it was homogenized and centrifuged firstly.
First, we included the weight of the blood pellet before it was homogenized.
75 ml of 20% sarcosyl in H2O was added to the sample and it was homogenized again.
Next, it was homogenized it and supernatant was obtained.
It was homogenized for 30 sec and subsequently subjected to ultrasonication for 5 min.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com