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We give an overview of FISH probe design, covering issues of affinity and specificity of probes, probe backbone options, cellular targets, and accessibility of target sequences.
The applications for molecules that bind proteins keep expanding, and issues of affinity, specificity, robustness, and time for development are in focus.
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These are not always captured by homology models, especially the issue of affinity.
Reported affinities range from nano- to millimolar dissociation constant (KD) values raising the issue of which affinity window is relevant for specific BRD-peptide interactions.
However, this technique depends on the use of antibodies to recognize the target protein of interest, and as with all techniques that rely on antibodies, issues of specificity and affinity arise.
However, its antibody-centric nature exposes it to similar challenges faced by other antibody-based procedures, of which the most prominent are issues of specificity and affinity in antigen recognition.
As described in another paper (Aloj et al., this issue), the affinity of all these 12 peptides was in the same low nanomolar range.
Mr. Byrne acknowledges a lack of affinity for the sitcom genre.
I think they took this as a sign of affinity.
D Coefficient of affinity.
Joyce Shabazz, Consulting Associate Senior Trainer / Director Of Affinity Caucus Programs.
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