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This study provides an alternative strategy to the difficult issue of transcript characterization.
The issue of transcript regulation during oocyte maturation is a controversial topic in developmental biology.
Data from a number of previous studies also showed that different OR genes, or even copies of the same OR transgene in different genomic locations, are expressed in different numbers of cells [ 41- 43], but these studies did not address the issue of transcript levels per cell.
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Moreover, in the case of poly-(A) RNA-Seq data on lower organisms, since coding regions of the genome are well separated from non-coding regions with lower activity, segmentation methods should allow the identification of transcribed genes as well as address the issue of new transcript discovery.
The method employed by Bellin and co-workers [ 23], whereby pyrosequencing of 3' cDNA ends and de novo contig assembly was used to create a library of unigenes for microarray design, represents an approach to transcriptome analysis that overcomes the issue of predetermined transcript data.
To address the issue of merged transcripts, this model allows a single transcript sequence to contain multiple genes, via the creation of a "middle UTR" state.
To resolve the issue of many transcripts with missing values across all time-points, STEM offers the option to set the missing value parameter.
That may be bad news for fundraising but it could be great news for galvanizing voters – especially with that issue of the speech transcript still hanging over Clinton.
You raised the issue, so just 'yes,' or 'no'?" "The only reason it's germane is because she brought the issue up, and said Donald Trump raised the issue of his Harvard transcripts.
In the Stewart case, the judge plans to hold the interviews in her private robing room and issue a transcript of each day's questioning on the next day, with jurors' names and possibly other private information blacked out.
The key issue of whether the antisense transcripts act as exquisitely specific gene regulators or are simply transcriptional waste that a cell has learned to live with is still a matter of controversy [ 2].
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