To address the issue of changes of the enzyme's hydration level as a possible cause for the observed low solvent storage stability, we incubated the lyophilized and the colyophilized powders in 1,4-dioxane with 0.5% (v/v) water added to the solvent and controlling the water activity using pre-hydrated BaBr2 salts directly added to the solvent.
"I've signed a contract that is 120 pages long that was also signed by Bill Gates and Steve Ballmer," he added, "and it includes ultimate issues of change of control".
The second issue is the pace of change of moving to "target" allocations.
"The key thing was to establish the issue of change as sort of a defining fault line in the debate," explained Michael D. McCurry, the former Clinton press secretary who is now Mr. Kerry's lead spokesman on the road.
Fully aware of these issues, when comparing the relative rate of change of the connectivity patterns in the insula and in the mPMC, we calculated the relevant measurements in each subject, hemisphere, and region of interest separately.
NHS employers which, with their staff, pay into the largest centrally run occupational pension scheme in Europe, were conciliatory about "the sensitive issue" of changes but warned threats of industrial action would damage patient care.
19 We considered only all cause mortality and did not examine the issue of changes in the underlying causes of death.
However, these studies are germane to gaining an understanding of broader issues of change in colonial and post-colonial periods.
They may throw up their hands in frustration or have tons of good suggestions, but you have to focus on the big issues of change as the rest of the situation smooths itself out.
Second, additional research efforts should be devoted to better understanding and improving the actual implementation of priority setting frameworks, particularly with respect to issues of change management and the resolution of impediments to action on recommendations for resource allocation.
