All the of IEA GO terms that do not have an associated ISS code were removed.
Coding of ISS and declaration of patient's outcome revealed a completion rate of 100% for both registers.
Because of the slightly different methods for coding the ISS within the two registries, however, a discrepancy of ± 9 points was tolerated.
The ISS evidence code was originally used for all annotations derived from sequence-based analyses and is appropriate when multiple kinds of computational evidence are used.
While every effort was made to code the injuries accurately, it is possible for some bias to exist as the ICD and AIS/ISS coding.
We assigned the chicken predicted proteins the GO annotations of human and mouse orthologs that are only based on direct experimental evidence codes (Table 1) and each chicken GO annotation was assigned an ISS GO evidence code, as per usual GO annotation procedure.
Larvae (12 LPG) from pig 1 were identified as T. britovi (TRC code ISS 333), whereas those from the blue foxes and the rats were found to be T. spiralis (TRC codes ISS 356-ISS 358 and ISS 359 respectively).
The GO annotations are generated by manual curation of the literature and by sequence similarity (GO evidence code ISS) using the GOanna tool followed by manual inspection of the alignments that are produced.
To compute the ISS, AIS injury codes are grouped into six body regions (head or neck, face, chest, abdominal or pelvic, extremities, and external) and the ISS is calculated as the sum of the squares of the highest AIS scores for the three most severely injured body regions.
