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Here, we investigate human MacroD1 by transition-state (TS) analysis based on kinetic isotope effects (KIEs) from isotopically labeled OAADPr substrates.
These effects of isotopic substitution are called kinetic isotope effects.
Primary isotope effects are often interpreted in terms of what is known as transition-state theory.
Scientists usually refer to the former as isotope effects and to the latter by a variety of more specialized names.
Both primary and secondary isotope effects decrease rapidly with increasing atomic number because the percentage difference in mass between isotopes tends to decrease.
Many other properties that depend on atomic motion, such as the thermal conductivity and viscosity of gases, manifest predictable isotope effects.
Kinetic isotope effects were used for further mechanistic analysis.
There are two types of isotope effects: isotope (exchange) equilibrium and the kinetic isotope effect.
However, up to now, no comprehensive compilation of N cycle isotope effects is available.
Isotope effects were found in the peak maxima and tail structures of these bands.
Thus, complex problems such as isotope effects on enzymatic reactions can now be examined, routinely.
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