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Experiments are conducted in 300 L unpressurized storage tank filled with initially isothermal water at 60 °C.
After amplification in an isothermal water bath for 70 min, samples containing IBDV generated the expected ladder-like products while other viruses generated no product.
The flasks were placed in an isothermal water bath shaker (Model Protech, Malaysia) at constant temperature of 30 °C, with rotation speed of 120 rpm, until equilibrium point was reached.
Then, the components were mixed by gentle stirring and vortex mixing using vortex mixer (REMI CM 101DX, REMI Equipment, Mumbai, India) and heated at 50 °C in an isothermal water bath to obtain a homogenous isotropic mixture.
The flasks were covered with aluminium foil and placed in an isothermal water bath shaker at constant temperature of 25 °C with rotation speed of 120 rpm for 24 h.
Consequently, it is believed that the colonisation of the deep sea may have been limited to periods and regions with an isothermal water column (Tyler, Young & Clarke, 2000).
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This paper addresses the synthesis problem of non-isothermal water networks using a mathematical programming approach.
Two submodels have been created to simulate non-isothermal water evaporation processes and pressure drop of two-phase flow through the porous medium.
This paper is an extension of our previous study [1] and addresses simultaneous synthesis of non-isothermal water networks heat-integrated with hot and cold process streams.
Synthesis of non-isothermal water networks consisting of water-usage, wastewater treatment, and heat exchanger networks has been recognised as an active research field in process systems engineering.
A recently proposed superstructure [2] for the synthesis of non-isothermal water networks is extended in order to enable additional heat integration options with process streams.
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