Sentence examples for isolation transformation from inspiring English sources

After blood draw standard protocols were followed for cell isolation, transformation or RNA extraction.

Microbiologic and molecular investigations (pulsed-field gel electrophoresis [PFGE], DNA isolation, isoelectric focusing analysis [IEF], PCR detection of resistance genes, plasmid isolation, transformation, and Southern analysis) were performed as described (2, 4, 7 ).

Following copy-number induction, performed as described above, and DNA isolation transformation of C. elegans N2 worms with fUL SB28 and plasmid pRF4 was performed by microinjection into adult syncytial gonads as described [ 5].

DNA isolations, transformations, electrophoration, restriction enzyme digestions, electrophoresis, ligations and hybridizations were performed using standard procedures [ 65, 74].

Plasmid-curing experiments and AmpC β-lactamase detection were performed on all the isolates and seven isolates showing the most common antibiotic resistance pattern were selected for plasmid isolation and transformation experiments.

Rice (Oryza sativa L. ssp. japonica) cultivar Nipponbare was used for protoplast isolation and transformation experiments.

Its isolation and transformation have thereby attracted increasing attention in recent times.

Polymerase chain reaction (PCR) amplifications, restriction enzyme digestion, agarose gel electrophoresis, plasmid DNA isolation, and transformation in E. coli were performed as described previously (Sambrook and Russell 2001).

DNA isolation, PCR, transformation of E. coli, DNA cloning and DNA analysis were performed using standard methods (Sambrook and Russel 2001).

Genomic and plasmid DNA isolation, manipulation, transformation and analysis were carried out by standard procedures [43].

Protoplast isolation and transformation was performed as described earlier [ 33].