Sentence examples for isolation step from inspiring English sources

To ensure that each mutant and control isolate was truly clonal, a second clonal isolation step was performed into 96-well plates to an average of 1 cell/1500 µl (i.e., 1 cell/10 wells).

In the isolation step, 14 colonies with different phenotypes were isolated from the air-exposed, pretreated CS samples and were named ZN1 to ZN14, respectively.

Note that the wet etch process is widely used in mesa isolation step for conventional III-V FETs.

By the coating of a 20-nm Al2O3 layer from the second isolation step, the channel height finally reached a total height of 25 nm.

So, it is often a key isolation step or a purification process in the synthesis of an active pharmaceutical ingredient (API).

In contrast to previous reports, this method does not require designing dye-labeled primers, and gel electrophoresis and isolation step after PCR were avoided in this homogenous method.

In parallel with the isolation of the Rosetteless mutant, we also co-isolated a wild-type strain (C2E5) that underwent all washing and clonal isolation steps but was not mutagenized.

A full bridge converter with high frequency transformer isolation, step-up the DC voltage level from the low voltage fuel cell along with poly phase boost converter, deliver required DC to the PWM inverter, which generate AC utility power output.

All of the isolation steps were performed in a single tube and were completed in no more than 35 min.

Such diligent analyses may offer an advantage for miRNA profiling and greatly reduce risk of misrepresentation from miRNA expression noise that typically results from additional serum or plasma isolation steps and sample contamination.

Whole blood offers advantages for miRNA profiling compared to other tissue types because it contains rich immune cell- and tissue-specific miRNAs with low risk of noise from additional serum or plasma isolation steps or sample contamination [12].