Your English writing platform
Discover LudwigSuggestions(1)
Exact(6)
During the MS3 analysis we used an online isolation specificity filter (Supporting Information).
We additionally employ an isolation specificity filter to minimize peptide coisolation (Ting et al., 2011 ).
Proteins were quantified by summing TMT reporter ion counts across all matching PSM after filtering based on isolation specificity (Pease et al., 2013).
MS3 precursors were fragmented by HCD prior to Orbitrap analysis (NCE 50, max AGC 1.5 × 10, maximum injection time 250 ms, isolation specificity 2.5 Th, resolution 30,000) (McAlister et al., 2012 ).
At the same time, we maintain the selectivity of the standard MS3 method by carefully defining the isolation notches of the SPS isolation waveform to ensure high isolation specificity of the target MS2 fragment ions.
The top ten precursors were selected for MS3 analysis, in which precursors were fragmented by HCD prior to Orbitrap analysis (NCE 55, max AGC 5 × 10, maximum injection time 250 ms, isolation specificity 0.5 Th, resolution 60,000) (McAlister et al., 2012 ).
Similar(54)
While there was a statistically significant difference in the sensitivity of these two cell lines for virus isolation, the specificity and PPV were very good (100 %, Table 1).
In this paper we describe the isolation and specificity of antibody fragments that can be used for the identification of Acanthamoeba.
This could relate to differences in experimental conditions, including patient sample engraftment heterogeneity, injection site, single cell isolation method, specificity of CLL markers used for detection, experimental time points and the mouse strain.
However, interferon-γ assays that use RD1 antigens in isolation may maximise specificity at the cost of sensitivity.
Results: DBS test was positive in all 72 congenitally infected babies and in four of the 437 negative at cytomegalovirus isolation (sensitivity 100%, specificity 99%).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com