DNA isolations, primer specifications, PCR amplification, DNA sequencing, voucher specimen information, GenBank numbers, and maximum parsimony phylogenetic analyses are described in Egan and Crandall [ 9].
Importantly, the X chromosome is enriched for genes that are known to play a role in reproductive isolation and specification in Anopheles.
A numerical algorithm solved the transient temperature field with consideration of the effects of cooling pipe specification, isolation of heat of hydration, and ambient temperature.
A given cell lineage in the developing embryo does not develop in isolation, and proper specification usually involves production of a signal for maintenance of itself and patterning of adjacent tissues [ 87].
Vendors quote tester specifications in isolation and under ideal conditions.
In New York, Goddard said there is now a widespread understanding in the agency of how these elements impact social isolation and community and new specifications and actions as a result.
"Total RNA" was prepared from embryos or adults by adding 4 volumes of TRIzol (Invitrogen), freezing in liquid nitrogen, followed by isolation according to manufacturer's specifications.
Take, for example, the sentence 'I am happy', considered in isolation from any particular utterance or specifications of who is uttering it at what time.
Blood samples were pooled and divided into 400 μL aliquots to determine qualification specifications of the RNA isolation procedure.
Total RNA was extracted from the frozen homogenized larvae by using TRI Reagent® (MRC, Inc ., and mRNA was extracted by using Promega PolyATtract® isolation system following the manufacturer's specifications.
