Sentence examples for isolation reverse from inspiring English sources

RNA isolation, reverse transcription (RT), regular PCR, and quantitative real-time PCR analysis were performed as previously described [34].

The pVAX2-rPGF-1 plasmid is a 3.4 kb vector encoding rPGF-1 which was constructed after isolation, reverse transcription and amplification of rPGF-1 mRNA from rat placenta in order to limit the effect of immune response.

Positive and negative controls for isolation, reverse transcription, and PCR were included in each run.

Total RNA isolation, reverse transcription, and real-time reverse transcription polymerase chain reaction (RT-PCR).

RNA isolation, reverse transcription, qRT-PCR reactions and data analysis were performed as previously described [ 30].

RNA isolation, reverse transcription, and RT-PCR were performed as previously described [ 20].

Procedures for RNA isolation, reverse-transcriptase polymerase chain reaction (RT-PCR), quantitative real time PCR analysis and primer sequences were previously described [20], [27], [29].

Total RNA isolation, reverse-transcription and quantitative PCR were performed as described above.

Technical noise is introduced at all stages in the processing pipeline from sample handling, cell-isolation, reverse transcription, cDNA amplification, sequencing and analytical stages.

Cells were collected after 1, 2, 3, 4, 5, 6 and 12 h in 1 ml Tri Reagent (Invitrogen) for total RNA isolation and reverse transcribed to cDNA by High-Capacity Reverse Transcription kit (Applied Biosystems, Carlsbad, CA, USA) according to the manufacturers' instructions.

hESC and hiPSC undergoing neural induction at various time points were collected and subjected to RNA isolation and reverse transcription by using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA), according to the manufacturer's protocol.