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On average, individual S. caementarium wasps yielded more morphotypes than C. californicum; however, this is likely because three sub-cultures were performed per C. californicum isolation plate versus eight per S. caementarium.
5 ml of molten Assay Top Agar (identical to assay agar, but containing 0.75% agar, 0.4% glucose, 0.02% casamino acids and 300 ng/ml doxycycline hyclate) at 40°C is mixed with a 50 µl thawed aliquot (∼5×107 cells per plate) of each strain of an assay pair, poured uniformly over the surface of a pre-warmed isolation plate, and allowed to solidify.
Scedosporium strains obtained from a single colony from the primary isolation plate from all patients were forwarded to the Molecular Mycology Research Laboratory, Westmead Hospital, for genotyping.
Given that a single colony is picked from the primary isolation plate and referred for susceptibility testing, additional mutants may have been found had multiple colonies been tested.
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The aim of this study was to identify bacterial isolates for potential use in the remediation of PAH-contaminated sites using selective isolation plating, Biolog™ MT2 evaluation and metabolic profiling on the Biolog™ system.
A set of genus-specific oligonucleotide primers developed for the rapid identification of unknown Amycolatopsis strains was used to determine whether representative strains taken from the selective isolation plates produced the diagnostic amplification product.
Isolation plates developed various types of colonies.
Several actinobacteria colonies were observed after incubation of isolation plates.
Isolation plates were incubated at 50°C for 24 h and then at 28°C for more than four weeks.
The Verrucosispora spp. and Micromonospora spp. appeared identical on isolation plates and provided a real world example of distinguishing strains cultivated from marine invertebrates.
In order to obtain directly colonies for further isolation, agar plate methods were preferred over MPN procedures with liquid media for determining concentrations of fecal bacteria.
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