Sentence examples for isolation of virus from inspiring English sources
The detection and isolation of virus in embryonated chicken eggs may help epidemiologic, genetic, and vaccine studies.
In past, the most common method was an isolation of virus in cell cultures but it was difficult, time consuming and lengthy process that requires experienced technicians.
Based on prior findings [33], [34], isolation of virus was attempted from feces suspended in RNAlater solution.
The large number of positive cells present in the MLNs at days 12 16 pi suggested that isolation of virus positive cells by FACSorting might be possible.
Isolation of virus was performed by inoculating the allantoic sac of four 9-day-old SPF ECE with the clarified BHI media containing the swab (0.25 mL per egg).
The viral etiology of cases was based on the isolation of virus (CPE and/or IFA/DFA positive) and/or a positive result by both RIT and RT-PCR For the genetic analyses of influenza viruses, viral RNA extraction was performed from the supernatant of infected MDCK cells using a QIAamp Viral RNA kit (QIAGEN, Valencia, CA) following the manufacturer's protocol.
A direct demonstration of the mechanism would entail isolation of virus-like particles containing two paired RNAs and demonstrating the RNA structure.
However, since isolation of viruses allows important detailed analyses of their antigenic, genetic and drug susceptibility characteristics, we recommend a combination of both techniques for influenza surveillance.
For questions of written informed consent for participation in the study, we refer to [ 1] and [ 2], where isolation of viruses from clinical samples is described.
Because the replication cycle of papillomaviruses can be completed only in differentiated epithelial cells, isolation of viruses from clinical samples is difficult 72.
