Sentence examples for isolation of viral from inspiring English sources

SBA-488 (20 µg/mL) was mixed with RSV-A2 stocks for 30 min at 37 °C before filtration and isolation of viral filament as described previously6.

A high-salt reagent composed of guanidinium thiocyanate, guanidine hydrochloride, urea, sodium citrate, and other compounds was designed for the single-tube isolation of viral nucleotides from body fluids.

This technology is primarily applied to the isolation of viral particles, subcellular organelles and fractions, and nucleic acids from biological samples.

VN/04 virus was used in animal challenge experiments and for the isolation of viral RNA.

We omitted MNase during the isolation of viral DNA to determine whether a similar phenomenon was occurring in our analysis.

The isolation of viral RNA is followed by reverse transcription of prescribed gene segments to generate cDNA, their subsequent amplification using primers to these targets, and the detection and/or sequencing of the amplificons.

After incubation at 27°C, viral supernatant was removed and diluted for isolation of individual viral clones.

We were able to observe the sequential establishment of closely related genotypes at two distinct population centers, and follow their differentiation due to relative isolation of the viral hubs over a period of several years.

For isolation of encapsidated viral nucleic acids, lysates were adjusted to 2 mM CaCl2 then incubated with 44 units of micrococcal nuclease (Worthington, Lakewood, NJ) to digest transfected plasmid DNA and unencapsidated HBV RNA.

Taking into account the relatively high percentage of sero-prevalence and the association of EHV-2 with respiratory disease [ 16], the isolation of this viral agent from horses showing different respiratory symptomathology was carries out.

During this time, diagnosis rests on isolation of the virus, viral RNA, or viral antigen from the specimen.