Sentence examples for isolation of scFvFc from inspiring English sources

One part was subjected to PCR isolation of scFvFc fragments.

For the detection and isolation of 11A scFvFc transduced cells, total mixed cells were blocked with 2% BSA for 30 minutes followed by staining with biotinylated GD03-Fc at an optimized concentration of 2.65 µg/ml (GD03-Fc protein was biotinylated using the EZ-link NHS-biotin, PIERCE) for 30 minutes on ice.

However, isolation of 11A-scFvFc expressing cells could not be reliably achieved at a mixing ratio below 1∶1000 (data not shown).

As a model for the isolation of rare antibody from a population of scFvFc expressing cells, 293T cells were transduced with lentiviruses encoding either 11A scFvFc or PS11 scFvFc at MOI of one.

To establish conditions for mammalian cell display of scFvFc antibodies, 293T cells were transduced with PS11-scFvFc-CD28-gp41-IRES-ZsGreen encoding lentiviruses.

Importantly, cell-surface expression of scFvFc was detectable at very low multiplicity of infection.

APC-conjugated anti-human Fc antibody (Jackson ImmunoResearch) was used to determine cell-surface expression level of scFvFc.

Treatment of transfected cells with sodium-chlorate, a sulfation inhibitor [15], [22], decreased expression but more significantly abolished sulfation of scFvFc proteins (Figure 3c lower and upper panels, respectively).

These viruses were analyzed for incorporation of scFvFc into the viral envelope and their capacity to bind specific antigens; and were further used to establish a mammalian cell display of surface-bound antibodies through transduction.

A human scFvFc-display master cell bank would serve as a rich source for screening and isolation of high affinity human scFv.

The isolation of the military outpost?