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Following the establishment of the germ theory of disease in the mid-1880s and the development of laboratory techniques for the isolation of microorganisms (particularly bacteria), the causative agents of many common diseases were discovered in rapid succession.
However, the efficiency of isolation of microorganisms that degrade solid forms of biodegradable plastic is low.
During the isolation of microorganisms, 313 microorganisms were chosen for further studies: 289 bacterial and 24 fungal strains.
Spread plate technique was followed for isolation of microorganisms through spreading 0.1 ml of the desired dilution to the surface of solid agar medium.
This study investigates the petrochemical contaminated site for isolation of microorganisms capable of assimilating PAH compounds mostly present at the petrochemical contaminate site.
This methodology is based on the isolation of microorganisms on agar plates containing selective growth medium and subsequent counting of colony-forming units.
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The work began with the traditional isolation of microorganism from environmental samples, and led to some interesting discoveries to synthesize chiral epoxides with varied structures that have expanded the scope of bio-epoxidation reactions.
Therefore, the isolation efficiency of microorganisms that degrade biodegradable plastic is low.
A metagenomic pyrosequencing-based approach for the isolation of cultivable microorganisms could accelerate the assessment of new enzymes and molecules and their possible application in biotechnological purposes.
This progress includes the isolation of extremophilic microorganisms for pollutant degradation in extreme conditions and the study of genes and enzymes related to biodegradation pathways.
Following the isolation of the microorganisms responsible for nitrification and nitrogen fixation, American soil scientists anticipated the time when farmers could 'seed' their crops and lands with these beneficial bacteria.
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