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This is the first report on the isolation of compound 3 from M. communis and the validation in the use of Egyptian herbal medicines against P. acnes.
In present study, qualitative chemical screening of aerial parts of V. odorata has led to isolation of compound 1 named as 3-(2′,4′,6′,6′-tetramethylcyclohexa-1′,4′-dienyl)acrylic acid, a new derivative of ionone, along with its antibacterial efficacy.
Fractions 40 60 were further purified by silica gel recolumn chromatography and the chromatography purified of these fractions led to the isolation of compound (500 mg).
Reaction time could be successfully shortened to 7 min and no sign of debromination was observed (entry 10) facilitating isolation of compound 6 in 77% of yield.
Fraction F3-5-4 (1.1 g) wappliedied to preparative HPLC system (mobile phase: CH3OH/H2O (70 : 30, v/v); flow rate: 5 mL min−1; UV detection at 210 nm) resulting in the isolation of compound 1 (36 mg).
Fraction F3-7-2 (0.2 g) wappliedied to preparative HPLC system (mobile phase: CH3CN/H2O (35 : 65, v/v); flow rate: 5 mL min−1; UV detection at 210 nm and 280 nm) resulting in the isolation of compound 3 (12 mg) and compound 4 (22 mg).
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A larger scale extraction was carried out in the next step in the allelopathic study, i.e., the isolation of compounds from the bioactive extract and chemical characterization by spectroscopic techniques, including NMR.
This is the first report of the isolation of compounds 1 9 (Fig. 1) from M. madagascariensis.
The dichloromethane fraction was subjected to further chromatographic separation for the isolation of compounds 1 13.
The extract was kept in the refrigerator (4 °C) ready for chromatographic isolation of compounds.
However, the isolation of compounds remains a challenging and a mammoth task.
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