Chickens lack lymph nodes (McCorkle et al., 1979) and lymphoid tissues with equivalent function are difficult to visualise and isolate, which makes the isolation of cells and analysis of local immune responses challenging.
For isolation of cells from xenografts, freshly isolated tumours were made into single-cell suspension as described (Varticovski et al, 2007).
Dielectrophoretic techniques allow for sorting, separation, and isolation of cells from different organisms and tissues.
PCR and FISH were performed on naturally contaminated samples and compared with the isolation of cells on selective media.
We thank the other members of the ImmGen Consortium, especially C. Benoist and T. Shay, for discussions; the core ImmGen team, especially A. Rhodes and K. Rothamel, for technical assistance; and A. Chicoine for assistance with the isolation of cells.
Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166low/negBMPR1B+ putative cartilage-committed progenitors.
Phage Emulsion, Secretion, and Capture (ESCape) is a novel micro-emulsion technology that utilizes water-in-oil (W/O) emulsions for the identification and isolation of cells secreting phage particles that display desirable antibodies.
In order to assess the suitability of the method, the assays were performed on naturally (n = 87) and artificially (n = 14) contaminated samples and results were compared to those obtained with the isolation of cells on selective media and with a PCR method.
This is achieved through the isolation of cells expressing a mesodermal marker, platelet-derived growth factor receptor-α (PDGFRA), following embryoid body (EB) formation.
It has been observed in several tissues that direct isolation of cells in serum-free media and on nonadhesive substrates results in the formation of spherical clusters of cells known as free-floating spheres.
This permits high-throughput processing and reduction in time required for isolation of cells.
