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However the vast majority of the clades containing photosynthetic sequences remain uncultivated emphasizing the need for a strong isolation effort.
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Secondly, I'd take on diplomatic isolation efforts.
In order to focus isolation efforts, selected glycerol stock cultures were screened to identify those with best growth and fermentation of xylose in the presence of ethanol.
This observation, along with the need for an H2-consuming partner, could guide development of growth media for future isolation efforts.
Surprisingly, alternative isolation efforts using, e.g., diffusion chambers with minimal amounts of host tissue or co-occurring microorganisms, or long-term incubation experiments have not yet been documented.
And yet, given that organ procurement for clinical purposes mandates that each organ procured pay an equal share of all costs required (∼25,000 USD for each organ procured) and if all 6,000 organs were procured for islet isolation efforts, the annual cost for those organs alone would be 6,000 times $25,000 USD or 150,000,000 USD.
Implementation of the case definition on the basis of nonspecific clinical features was particularly challenging in areas with community transmission, since the ability to focus surveillance and isolation efforts on persons with epidemiologic links to specific travel or contact with other SARS patients was no longer appropriate.
And following in the war's train was the full array of afflictions, disorders, and distortions of the Presidency which seemed to be indivisibly bound up with the war effort: isolation, secrecy, deception, rage, repression.
Neighboring countries have responded by pushing harder to end Myanmar's international isolation, including an effort to lift the economic sanctions imposed by the United States, the European Union and other Western countries.
The bookseller, Lam Wing-kee, went public in June about the five months he spent in detention in mainland China, saying that he had been held in isolation in an effort to force him to disclose the names of mainland buyers of gossipy political books published in Hong Kong by the business that employed him.
In addition, the PCR from diluted boiled spot tests worked remarkably well allowing subcluster identification in the initial stages of mycobacteriophage isolation with minimal effort.
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