Sentence examples for isolation construction from inspiring English sources

Following a period of relative isolation, construction of the Karakoram Highway and the ensuing development activity have been catalysts for rapid infrastructural and social change over the last two decades.

RNA isolation, construction of normalized cDNA Library, plasmid DNA preparation, automated DNA sequencing, assembly, annotation, validation of ESTs and other bioinformatics analysis were directed by PM and carried out by PN.

QF conducted the major part of this study including experimental design, sampling for RNA isolation, constructions of the subtractive cDNA libraries, EST analysis, RT-PCR analysis, and manuscript preparation.

The Trβ2 promoter isolation, clone construction, and the general expression were described previously.

RNA isolation and construction of cDNA library PT033 (isolated haustoria) was according to Li et al. [ 50].

Detailed methods describing DNA isolation, library construction, template preparation, automated cycle sequencing and metagenomic assembly can be found in Rusch et al. (2007) [41] and Venter et al. (2004) [40].

PP did PBMCs isolation and construction of Nck1-shRNA plasmid.

Total RNA isolation, library construction and Illumina sequencing were performed according to Zhang et al. [ 74].

MK, SK and RR contributed to RNA isolation, library construction, template preparation, sequence determination and assembly.

Total RNA isolation and construction and sequencing of cDNA libraries were performed by Vertis Biotechnologie AG (Germany).

Bioinformatics analyses: 1. miRNA identificationSmall RNA isolation, library construction and sequencing are described in Subramanian et al.[ 18].