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Virus isolation conducted for select tissue samples showed LASV titers of 5 7 lof10 tissue/g of tissue; blood samples collected on day 10 and at the time of euthanasia (5 and 6.25 log10 TCID50/mL, respectively) showed viremia.
Similarly, virus isolation conducted on all samples, including those RT-PCR negative foRT-PCR negative), was successfor only for animals <4 years of age but particularly for calves.
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RNA isolation was conducted using the RNA isolation kit NucleoSpin RNA XS (Macherey and Nagel).
Thus, further isolation was conducted using S14-2 and S14-3, wereh were oily and yellow.
For the evaluation of the CAN router and its proposed means for fault detection and isolation we conducted experiments.
A study of several critical aspects of six-axis hexapods used for space-based vibration isolation is conducted.
The isolation was conducted following the methods described by Uddin et al. (2011) where spread and pour-plate techniques were adopted.
Nucleotide pool isolation was conducted as described in [26].
RNA isolation was conducted according to the Trizol manufacturer's instructions.
RNA isolation was conducted on infected and non-infected cell cultures at the indicated time-points using the commercially available Absolutely RNA Microprep Kit from Stratagene.
Slower dissemination could be a contributing factor for the negative heart culture, when bacterial isolation was conducted within 1 month post-inoculation.
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