Sentence examples for isolation column from inspiring English sources

For this, DNA was isolated from suspected colonies by a QIAamp DNA isolation column (Qiagen, Holden, Germany) according to the manufacturer's recommendations, including 10 min incubation at 55°C with proteinase K.

CD4 T cells were isolated from the spleen using the CD4 T cell isolation column from MACS.

The CCC was used as the first dimensional isolation column, where an environmental friendly polar two-phase solvent system of isopropanol and 16% sodium chloride aqueous solution (1 1.2, v/v) was introduced for low toxicity and favorable resolution.

By applying a vacuum to draw the solution through the packing, product separation from hydrophobic and hydrophilic impurities is accomplished in a two-stage purification unit: a short pre-column functions as a trap for hydrophobic impurities, while a second, longer SPE column is used as a product isolation column.

Thus, under ideal SDC conditions, washing with a 100% aqueous solvent will achieve retention of hydrophobic impurities on the trap, with displacement of product and hydrophilic impurities from the trap to the product isolation column; hydrophilic impurities are thus displaced off the product isolation to waste, leaving only product retained on the main column.

DNA was purified using Omega EZNA® DNA isolation column (Omega Bio-Tek, Norcross, GA).

The RNA samples were treated with DNAseI and purified using the Qiagen RNA isolation columns.

Total RNA was isolated from FG-activated, FBR and blood CD68+ cells using Trizol (Invitrogen) and RNeasy Mini RNA isolation columns (Qiagen, Hilden, Germany).

Allogeneic T lymphocytes were isolated from PBMC of a single healthy volunteer by nylon fibre non-adherence using T lymphocyte isolation columns (Nylon Fiber Column T, Wako, Japan).

The RNA containing aqueous layer was diluted in binding buffer and applied to Qiagen (Venlo, Limburg, Netherlands) or Denville (South Plainfield, NJ) RNA isolation columns.

Ethanol (210 μl) was then added and the lysate was transferred into DNA isolation columns, which were then centrifuged at 16 000 × g for 1 min and then sequentially washed with the two proprietary washing buffers.