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Isolation attempts in newborn mice and Vero cells from the samples yielded virus isolates from five patients.
All virus isolation attempts were unsuccessful.
Nevertheless, viral isolation attempts were negative, with no anti-Morbillivirus antibodies being detected in serum.
Nevertheless, no anti-Morbillivirus antibodies were detected in serum, humor aqueous or cerebrospinal fluid and virus isolation attempts failed.
Nevertheless, no anti-Morbillivirus antibodies were detected in serum, humor aqueous or cerebrospinal fluid and virus isolation attempts were all negative.
Not all of the isolation attempts have been successful, revealing stable co-cultures.
Most isolation attempts from segment bark in Study 1 yielded cultures of different fungi at frequencies varying between 59%and80%0% over all periods after treatment.
Results are shown in Figures 1 and 2. Figure 1 Percentage of isolation attempts from bark cores yielding cultures of Phytophthora kernoviae at intervals after treatment of pine segments with Phytophthora kernoviae oospores and incubation at five temperatures (Study 1).
Figure 2 Percentage of isolation attempts yielding cultures of Phytophthora kernoviae after application of oospores to the bark surface for heat treated and untreated oospore suspensions in Study 1 (back transformed means with standard errors).
As in Study 1, isolation attempts after application of zoospores of both species in Study 3 did not yield cultures of either Phytophthora kernoviae or Phytophthora pluvialis following incubation at any of the temperatures tested for periods from 24 96 hours.
Virus isolation attempts and antigen detection tests on the same liver/spleen organ extracts were negative, which along with the quantitative PCR data, indicate low levels of Marburg virus in these organs.
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