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When no influenza A virus was detected upon the initial virus isolation attempt, the allantoic fluid was passaged once more in embryonated chicken eggs.
On primary isolation, attempt was made to pick one colony of each morphotype, when more than one was present.
When no influenza A virus was detected on the initial virus isolation attempt, the allantoic fluid was passaged once more in embryonated hens' eggs.
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Isolation attempts in newborn mice and Vero cells from the samples yielded virus isolates from five patients.
Not all of the isolation attempts have been successful, revealing stable co-cultures.
Most isolation attempts from segment bark in Study 1 yielded cultures of different fungi at frequencies varying between 59%and80%0% over all periods after treatment.
Results are shown in Figures 1 and 2. Figure 1 Percentage of isolation attempts from bark cores yielding cultures of Phytophthora kernoviae at intervals after treatment of pine segments with Phytophthora kernoviae oospores and incubation at five temperatures (Study 1).
Figure 2 Percentage of isolation attempts yielding cultures of Phytophthora kernoviae after application of oospores to the bark surface for heat treated and untreated oospore suspensions in Study 1 (back transformed means with standard errors).
As in Study 1, isolation attempts after application of zoospores of both species in Study 3 did not yield cultures of either Phytophthora kernoviae or Phytophthora pluvialis following incubation at any of the temperatures tested for periods from 24 96 hours.
Since only one strain of bacterium is expected to be associated with a given ant colony [cf. 39], isolation attempts were done until a Pseudonocardia strain was obtained; this meant performing isolations on between one and ten ants per colony.
Virus isolation attempts and antigen detection tests on the same liver/spleen organ extracts were negative, which along with the quantitative PCR data, indicate low levels of Marburg virus in these organs.
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