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We then present designs and outcomes for microfluidic devices utilized thus far for different steps in the IVP process: gamete isolation and processing, fertilization, and embryo culture.
RNA isolation and processing: a total of 192 μg of RNA was isolated from 5×10 cells using the RNeasy kit (Qiagen), including DNase treatment, and followed by mRNA isolation in two rounds of Poly(A) Purist (Ambion), according to the manufacturers' protocols.
First, as a product for biomedical use, BMSS should be a unitary and reproducible material, and this can be assured by controlling the extent of chemical degradation during its isolation and processing.
RNA isolation and processing were performed as described.
RNA isolation and processing was performed as previously described with minor modifications [ 26].
Since mRNAs are biological materials of transcriptomes, protocols for RNA isolation and processing are critical for transcriptome studies.
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This property is quite useful during isolation, purification and processing of antimicrobial compounds from commercialization point of view.
In our study, both the Reischl et al. protocol and FTA® Elute were, indeed, fast and easy to use by single-step isolation procedures and processing times of 40 and 110 min for 42 samples, respectively.
Bilateral L4 and L5 DRGs were harvested from each cohort, yielding a total of 40 samples for RNA isolation and TLDA processing.
EA assisted with the tissue isolation and tissue processing.
These results suggest that the differences may result from the different technical platforms, glomerular isolation protocols, normalization and processing of the raw data as well as different categorization criteria.
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