We determined lateral phase separation temperatures by measuring the temperature dependence of fluorescence from trans-parinaric acid inserted into liposomes prepared from isolated membrane phospholipids.
To isolate membrane proteins that bind to salusin-β, we immobilized both C- and N-terminal salusin-β to NHS Sepharose 4B gel and ECH Sepharose 4B gel, respectively, since the N-terminal 3 5 amino acids may undergo cleavage upon binding.
To investigate whether sGRP78 is also accompanied or facilitated by co-chaperones in beta cells, we isolated membrane fractions from control and cytokine-exposed INS-1E cells by immunoprecipitation with an anti-GRP78 antibody.
As is known, the isolated membrane cytochrome CYP11A1 is able to form oligomers in solution [8].
The morphology of membrane specializations of the cortex and nucleus of bovine lenses has been analyzed for both isolated membrane fractions and intact tissue fragments.
This finding validates the use of uncompetitive antagonist binding as a measure of the functional activation of the NMDA receptor-coupled ion channel in isolated membrane preparations.
The ProteoJET™ membrane protein and cytoplasmic/nuclear protein extraction kits (Fermentas GmbH, St. Leon-Rot, Germany) were used to isolate membrane, cytoplasmic and nuclear proteins.
Consistent with previous work [13], [19] [21], we observed isolated membrane fragments and non-synaptic organelles, such as free mitochondria, in addition to intact synaptosomes (Fig. 1A).
The location of SynK in cyanobacteria in both thylakoid and plasmamembranes was revealed by immunogold electron microscopy and Western blotting of isolated membrane fractions.
Compared to no treatment (CON), S1P + GF stimulation enhanced MT1-MMP levels in isolated membrane fractions.
