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Three pulsotypes were found after characterizing the isolates with PFGE.
majus and group B isolates with M. nivale var.
Only isolates with phenotypic characteristics representing Fusobacterium could be cultured.
coli isolates with melt peaks consistent with those stated above.
Four clusters were distinguishable: cluster I grouped five isolates with a PCR product of 440 bp, cluster II 32 isolates with a PCR product of 600 bp, cluster III 12 isolates with a PCR product of 720 bp and cluster IV 44 isolates with a PCR product of 740 bp.
From the different samples obtained, 340 isolates with the morphology typical of yeast were obtained.
Besides the herd pulsotype, a minority of isolates with other pulsotypes were found on the carcasses.
Fig. 4 The virulence comparison of F. asiaticum isolates with different genotypes or SCAR types.
Each cluster consisted of at least four isolates with a similarity level of at least 65%%.
In contrast, extraction at 70 °C generated isolates with lowest glucose content.
The fungal isolates with decolorizing activity and their level of decolorization are reported in Table 2.
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