Attachment to egg shells varied by time (1 or 20 min) and temperature (4, 22 and 42°C), with S. Typhimurium isolates attaching at higher levels (P < 0.05) than S. Sofia after 1 min at 4°C and S. Typhimurium ATCC 14028 attaching at higher (P < 0.05) levels at 22°C.
It was shown for the first time that freshly isolated chondrocytes attached well to the chitin scaffold and synthesized an extracellular matrix similar to that found in other cartilage tissue engineering constructs.
In the constructs, consisting of cartilage explant and chondrocyte pellet, isolated chondrocytes attached to the surface of the explant at the beginning of the culture, followed by significant chondrocyte death at the interface between chondrocyte pellet and explant.
A Dynal MPC® magnet was used to isolate beads attached to the unwanted cells.
Retinas were isolated and attached on black Millipore filters (AABP02500, Millipore, Billerica, MA, USA) with ganglion cell side up.
A 1 2 mm wide section (from rib to tendon) of diaphragm was isolated and attached to a force transducer.
Freshly isolated hepatocytes attached to collagen-coated dishes after initial plating and formed semi-confluent monolayers within 48 h.
All experiments were carried out at room temperature using freshly isolated cells, attached to the poly-L-lysine coverslips of the perfusion chamber.
42, 43 A 1 2 mm wide section (length from rib to tendon) of diaphragm was isolated and attached to a force transducer.
After sedating the animals with intramuscular ketamine (50 mg/kg) and xylazine (5 mg/kg), hearts were isolated and attached to the perfusion apparatus as described in more detail by Vanagt et al. [ 40].
