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Ploegh envisions the technique being used to create a new type of personalized therapy in the future your own cells could be isolated, used to create stem cells that differentiate into erythroblasts, genetically modified to carry a molecule, and reinjected into your body.
Cross-linking was reversed at 65°C overnight and DNA isolated used a QIAquick PCR purification kit (Qiagen).
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Total RNA was isolated using the miRVANA miRNA isolation kit.
The compounds were isolated using chromatographic techniques.
Organisms were isolated using two different techniques.
The dioxetane thus formed was isolated using column chromatography.
EVs isolated using microfluidic devices tend to retain their morphology.
White blood cells (WBC) were isolated using Ficoll-Hypaque technique.
DNA was isolated using CTAB method (Raeder and Broda 1985).
LAB were isolated using MRS and M17 media.
The RNA was isolated using chloroform extraction and isopropanol precipitation.
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