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Quadruple auxotrophic strains (niaD− sC−ΔargB adeA− or niaD− sC−ΔargB adeB−) were subsequently isolated upon UV mutagenesis of the triple auxotrophic strain (niaD− sC−ΔargB) followed by screening of red-colored colonies for adenine auxotrophy.
Included patients (screened for HIV) had two sputum smear samples positive for acid-fast bacilli and with M. tuberculosis isolated upon culture.
The homozygous mutant mESC lines for exon 3 deletion were isolated upon selection of heterozygous mutant cells with high dosages of G418, which may induce chromosome loss in ES cells.
All 10 patients were isolated upon MRSA identification.
The extracellular part of CAgp130 was isolated upon XhoI and EcoRI digestion of pcDNA5/FRT/TOspecial-CAgp130-YFP pcDNA5/FRT/TOspecial-CAgp130-YFP pcDNA5/FRT/TOspecial-CAgp130-YFP
Primary E13.5 MEFs were isolated upon timed mating of Rac-FRETKi/fl mice and cultured for up to 15 days.
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We first determine the general impacts of a large-scale disturbance associated with tropical cyclone Heta, and isolate upon four sites where similar coral reef assemblages existed, but differential dynamics through time were noted.
The ESBL activity of K. pneumoniae clinical isolates collected from both community and hospital settings is concordant with previous reports from Egypt and other countries which indicate faecal carriage of these pathogens in the community and may have important implications as a risk factor for acquiring ESBL-producing K. pneumoniae isolates upon hospital admission.
Although the ECD is not present in the crystal structure of mGluR1 dimer (4OR2) (Wu et al., 2014), conformational changes of the dimerized, isolated ECDs upon agonist binding have been observed in previously reported crystal structures (Kunishima et al., 2000).
The fraction of biotinylated recycled proteins was isolated similarly upon subsequent recycling of internalized proteins to the cell surface for 45 min at 37°C.
One such potent TLR agonist is an Eimeria tenella expressed antigen, originally isolated based upon its ability to induce high levels of IL-12 in bovine intestine [11].
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