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Eyes were enucleated and retinas isolated two weeks after the subretinal injection.
Most cells did not survive the HL conditions (Table 1), but a limited number of single green colonies appeared and were isolated two weeks after the onset of the selection.
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Primary full-length env genes were PCR amplified from genomic DNA isolated two week post-inoculation from the last recipient (ROm-8) and cloned into the expression vector pcDNA6/B.
PBMCs isolated two, four and 52 weeks post vaccination were stimulated with H1-antigen at a concentration of 0.5 µg/ml.
PBMCs isolated two, four and 52 weeks post vaccination were stimulated with 5 µg/ml H1 for 1 h in the presence of 1 µg/ml anti-CD28 (clone 37.51) and anti-CD49d (clone 9C10 (MFR4.bothboth from BD Pharmingen) and subsequently incubated for 5 6 h at 37°C following the addition of 10 µg/well brefeldin A (Sigma-Aldrich) and 0.7 µl/well monensin/GolgiStop (BD Pharmingen).
She isolated two individual strands.
They were challenged with RHDV-TP isolate four weeks after last boost immunization.
Prostate glands were removed from 243 adult specimens that had been sexually isolated for two weeks and had a shell length between 26 and 30 mm.
For this test we used a total of 30 snails with a shell length of 26 to 30 mm that had been isolated for two weeks.
Total RNA was isolated from two week old rice plants exposed to cold stress (4°C) for 2 hours using Trizol reagent according to manufacturer's instruction (Invitrogen, UK).
Total RNA was isolated from two week old seedlings (both Col-0 and npr1-1) in two independent biological replicates using Spectrum plant total RNA Kit (Sigma-Aldrich) according to the manufacturer's instructions and eluted with nuclease-free water.
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