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The sequences obtained from the 6 isolated transcripts were compared against those present in the databases.
Furthermore, a significant part of the isolated transcripts may be non-coding RNAs.
Aplysia G proteins encoded by previously isolated transcripts from rhinophore sensory epithelium [ 29] are shown schematically in Figure 9a.
Transcript expression validation by quantitative real time PCR and ISH technique demonstrated that these isolated transcripts displayed a polar distribution in the two-celled proembryos.
RNA-seq method involves the conversion of isolated transcripts into the complementary DNA (cDNA), which is then directly sequenced in a massively parallel deep-sequencing-based approach [ 18].
Furthermore, analogously to the PIN clones herein isolated, transcripts of some PINs were found to increase following auxin application in poplar, pea and A. thaliana [ 60, 64, 65].
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Sequencing of the isolated transcript showed that it was a perfect match to the antisense strand of FXN, and BLAST analysis of this sequence showed only a single hit (to the FXN locus), indicating that FAST-1 originates from the FXN locus.
mutation (3' S.J. of intron V) on gene transcription, primers spanning exons II to IX were used to isolate transcripts from the SBEIIa-B -2 mutant.
We used the mRNA-tagging strategy to isolate transcripts specifically from C. elegans larval neurons.
We then used parametric testing to isolate transcripts differentially expressed between the tissues.
Strains used to isolate transcripts via mRNA-tagging were N2 (wildtype Bristol strain) and SD1241 (gaIs153, F25B3.3::FLAG::PAB-1) [ 8].
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