Sentence examples for isolated to perform from inspiring English sources

Cells were harvested and total RNA was isolated to perform cDNA synthesis using a SMART-IIA primer (#797, Table 5) and the first gene-specific primer (#375 for fadD1 and #372 for fadD2, Table 5).

To determine whether this inhibitory effect of melatonin over aromatase activity was due to a downregulation of the aromatase expression at the transcriptional level, we then incubated glioma cells with either melatonin (1 m M, 1  μ M or 1 n M) or vehicle for 90 min and total RNA was isolated to perform real-time PCRs with specific primers for rat aromatase.

We then used the whole antigen from an outbreak isolate to perform IgM and IgG Western blots on the first and second serum samples from researcher 3: results were negative.

Referral bias is the most probable reason for the higher proportion of MDR TB in our microbiology laboratory, as our laboratory receives isolates to perform DST for second line antituberculous drugs and also receives specimen from hospitals specialised in TB treatment.

Using the fission yeast Schizosaccharomyces pombe, we present a method whereby cell-ghosts containing functional contractile rings can be isolated and used to perform various biochemical analysis as well as detailed electron microscopy studies.

As a first step, we harvested transgenic embryos at embryonic day 13.5 and isolated total RNA to perform quantitative RT-PCR to confirm increased expression of miR-130a.

RNA from the shRNAi clone, mock, and TC71wt from the early and late stages was isolated and used to perform duplicate hybridisation experiments with the Affymetrix HG-U133A platform.

The methyl red test was conducted to verify the ability of isolate RH5 to perform mixed acid fermentation (Palitzsch 1911; MacFaddin 1980); 100 µL of inoculum of isolate RH5 was inoculated in a tube containing MRVP (methyl red Voges Proskauer) broth and incubated at 35 °C for 24 h.

We estimate that the per isolate cost to perform MLST using HRM is 20 30% that of sequencing.

Cells were harvested 48 h after transfection to isolate RNA and to perform immunoblots.

In part, this relates to the difficulty of synthesizing or isolating sufficient material to perform detailed studies.