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To assess the validity of the approach, HEK293 (ATCC) cells were infected with these viruses, and individual cells were isolated to develop clonal cell lines expressing either the miR-132 inverse reporter or the reporter control.
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To address this, we evaluated the ability of individual clinical isolates from a diverse collection of infecting isolates to develop biofilms using the semi-quantitative 96-well plate assay as described [ 30, 34, 35].
But these issues are not isolated to the developing world.
This disparity does not appear to be isolated to one developed nation.
Twenty L4-stage wofms of each genotype were isolated and allowed to develop 30 hr at 20°C into gravid adults.
Similarly, there was a greater chance for the individuals starting with 2-component state and the isolated hyperglycemia state to develop MS than the others.
In 1000 ppm O2, isolated embryos continue to develop and die with gross morphological and developmental defects (Nystul et al., 2003).
We systematically reviewed the highest level of available evidence on the nonoperative and operative treatment of isolated patellofemoral osteoarthritis to develop an evidenced-based discussion of treatment options.
Second, several phases of colonization and mixing among islands occurred before the bananaquit populations became isolated long enough to develop reciprocal monophyly in mtDNA.
Our findings collectively suggest that, in addition to isolation of gallic acid, various other active constituents of TD should be isolated and characterized to develop it as a potential therapeutic agent for treating hepatocellular carcinoma.
Because it is well known that people with perceived stigma may have a low self-image and be socially isolated and predisposed to develop psychological distress (32), further research is needed in this area with a large sample size and strong epidemiological study designs.
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