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We isolated the zebrafish mych (myc homologue) gene.
Thus, we had isolated the zebrafish cfl1 gene by PCR cloning from zebrafish embryonic complementary DNAs.
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L. hongkongensis was isolated from the zebrafish that died.
pcDNA4.ZfCRY4 was constructed by inserting ZfCRY4 cDNA, which was amplified by RT-PCR from total RNA isolated from the zebrafish embryonic Z3 cell line (8), into pcDNA4/myc-his (Invitrogen) in frame with a Flag tag at the C-terminus.
We isolated the upstream genomic DNA of zebrafish PP1 (~5.3 kb) and PP2 (~6.7 kb) and introduced green fluorescent protein (GFP) and red fluorescent protein (RFP) into zebrafish under the upstream sequence, respectively.
L. hongkongensis was re-isolated from the zebrafish that died.
To isolate the full length zebrafish lamin A/C cDNA, the zebrafish lamin A mRNA sequence deposited in GenBank was used (accession number AF397016) [82].
In a forward genetic screen for zebrafish craniofacial mutants, we isolated the b1075 mutant allele.
We isolated zebrafish s1pr5a from a zebrafish cDNA library (AB strain) by PCR using the primers zS1PR5a-S and zS1PR5a-AS (supplementary material Table S1).
Through a forward genetic screen for craniofacial mutants, we isolated a zebrafish mutant in which the first cysteine of the second zinc finger of Gata3 is mutated.
To explore the biological functions of the cullin box domain of ankyrin repeat and SOCS-box containing protein 11 (d-Asb11), a key mediator of canonical Delta-Notch signaling, we isolated a zebrafish mutant lacking the Cul5 box (Asb11Cul).
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